Random Access 96 Well Skirted Flat Bottom PCR Plate, 2D Coded

96 individual tubes in plate format, low profile, flat bottom 2D coded, 0.1 ml clear polypropylene tubes, white rigid polycarbonate frame, linear barcoded, cut corner H1, working volume: <100 µl, total well capacity: 180 µl

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Overview

96 individual tubes in plate format, low profile, flat bottom 2D coded, 0.1 ml clear polypropylene tubes, white rigid polycarbonate frame, linear barcoded, cut corner H1, working volume: <100 µl, total well capacity: 180 µl

  • 96 well, fully skirted, low profile format with each tube inserted separately into the plate frame, allowing for selection and removal of individual tubes from the plate
  • Each tube clicks into place in the frame ensuring tubes are secure once inserted
  • We have now extended our Random Access range to include PCR plates with 2D coded flat bottoms for applications that require superior sample tracking
  • In applications where it is essential for samples to be tracked throughout their processing, e.g. for diagnostic assays on clinical samples, tube labeling is a far safer and reliable method compared to cap or seal labeling (which can be misplaced, damaged or rendered unreadable after piercing)
  • Our plates have a unique 2D code printed on the bottom of the well and a linear barcode on the side of the plate to allow for identification of both the plate and individual tubes within
  • The rigid polycarbonate skirt of this design eliminates warping and distortion during PCR
  • Together with the SBS footprint, this makes the plate suitable for use with robotic systems, ideal for both small and high throughput labs
  • Empty frames are available for tubes to be transferred to
  • The frames can be re-used multiple times, depending on the application
  • Sample tubes can be identified by the unique 2D code on the base of the wells, removed from the Random Access plate quickly and easily, and moved to another Random Access frame for downstream processing or storage
  • Quick selection of desired tubes reduces the time needed for the sample plate to be out of the freezer and therefore reduces the chance of other wells defrosting in the meantime
  • Flat bottom PCR plates can be stacked, allowing for optimal use of freezer space as multiple plates can be housed in the same space as a storage rack
  • The 2D codes on the bottom of the wells can be read by most 2D datamatrix readers including FluidX Whole Rack Scanners
  • The codes are highly scratch resistant and can withstand cold storage (-80 °C), temperatures up to 100 °C and solvents such as DMSO
  • 2D datamatrix codes utilize data redundancy so even if codes are partly destroyed, the information will still be retained
  • Each code is tested for readability and guaranteed to be unique
Figure 1. Technical drawing of a conical RA PCR tube (A) compared to a flat bottom RA PCR tube (B)
  • Figure 1 illustrates the differences in tube format between the standard conical PCR tube and the flat bottom tubes
  • The surface area of the optical base is 7.74 mm2, with a thickness of 0.5 mm
  • The total volume of a conical well is 0.2 ml compared to 0.18 ml for flat bottom tubes
Key Features
  • Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen

Tubes

  • Individually removable from the rigid frame, while the rest of the tubes can remain in storage, reducing freeze-thawing of the whole plate
  • Thin walled tubes are made from PP for optimal results in (q)PCR whilst also being suitable for storage and incubation
  • <100 µl working volume, <180 µl total well capacity

Frame

  • Rigid polycarbonate frame provides mechanical stability of the plate
  • These plates are labeled with linear Code 128-format barcodes to aid identification and traceability for your samples
  • Alphanumeric grid reference to aid well and sample identification

Use

  • Suitable for small volume sample storage, allowing for optimal use of freezer space as multiple plates can be housed in the same space as a storage rack
  • Ideal for use with robotic systems
  • Fewer transfer steps as the same tube can be used for microscopy, (q)PCR and storage
  • Each tube can be individually heat sealed using the Random Access Pierce Heat Seal Strong, such as the contents of each tube is left secure from cross contamination and evaporation for storage and transfer
  • Each tube can still be separated after sealing

Options

  • Also available as a Random Access optical bottom plate (4ti-0970/RA) with black frame, ideal for microscopy and PCR
  • Empty frames are available for tubes to be transferred to; frames can be re-used multiple times, depending on the application
  • We also offer customization of the Random Access Plates such as color coding of the individual wells; please contact us for details
  • The plate can be sealed in one step using Random Access Pierce Heat Seal Strong (4ti-05381/RA) on the 4s3™ Semi-Automatic Sheet Heat Sealer (using the 4ti-0613 Random Access adapter), resulting in individually sealed tubes with pierceable seals to allow for sample access
Specifications

Technical specifications

Parameter Value
Plate length 127.76 ± 0.25 mm
Plate width 85.48 ± 0.25 mm
Plate height 15.50 ± 0.25 mm
Well depth 12 ± 0.10 mm
Well diameter 5.46 ± 0.10 mm
Distance to center of A1 from top edge 11.24 ± 0.25 mm
Distance to center of A1 from left edge 14.38 ± 0.25 mm
Pitch (distance between A1 and A2) 9.00 mm
Code
Size 12 x 12 px; 2 x 2 mm
Format white on black
Content 8 digit numeric
Compatibility
  • AGILENT/STRATAGENE
    • Thermal Cyclers (96 well block): Robocycler Gradient
    • qPCR Cyclers (96 well block): AriaMx
  • BIO-RAD
    • Thermal Cyclers (96 well block): C1000 Touch | S1000
    • qPCR Cyclers (96 well block): CFX96 Touch | CFX96 Touch Deep Well | CFX connect
  • BIO-RAD MJ RESEARCH
    • Thermal Cyclers (96 well block): PCT100™/200™/220™/221™/225™/240™
    • qPCR Cyclers (96 well block): Chromo4™ | Opticon2™
  • EPPENDORF
    • Thermal Cyclers (96 well block): MasterCycler® ep/ep gradient/Pro/Pro S/nexus/nexus gradient/nexus SX1/nexus GSX1
    • qPCR Cyclers (96 well block): MasterCycler™ ep realplex
  • PEQLAB/VWR
    • Thermal Cyclers (96 well block): peqSTAR 96X
  • SENSOQUEST
    • Thermal Cyclers (96 well block): Labcycler
  • TECHNE
    • Thermal Cyclers (96 well block): TC412 | TC512 | Genius | Genius Quad | Touchgene | Touchgene Gradient | Flexigene
    • qPCR Cyclers (96 well block): Quantica

For recommended plate options depending on manufacturer, block type, and instrument please refer to the 4titude Plate Instrument Compatibility Table.

Ordering

Product codes

Part number Description
Random Access 96 Well Skirted Flat Bottom PCR Plate, 2D Coded
96 individual tubes in plate format, low profile, flat bottom 2D coded, 0.1 ml clear polypropylene tubes, white rigid polycarbonate frame, linear barcoded, cut corner H1, working volume: <100 µl, total well capacity: 180 µl
4ti-0975/RA clear tubes, white frame; 50 plates
4ti-0975/RA-F* white frame (no wells, frame only); 10 frames

*Please note, /SBC must be added to 4ti-0960/RA-F to include a standard single barcode