Random Access 96 Well Skirted Optical Bottom PCR Plate

96 individual tubes in plate format, low profile, flat optical bottom, 0.1 ml clear polypropylene tubes, black rigid polycarbonate frame, cut corner H1, working volume: <100 µl, total well capacity: 180 µl

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Overview

96 individual tubes in plate format, low profile, flat optical bottom, 0.1 ml clear polypropylene tubes, black rigid polycarbonate frame, cut corner H1, working volume: <100 µl, total well capacity: 180 µl

  • 96 well, fully skirted, low profile format with each tube inserted separately into the plate frame, allowing for selection and removal of individual tubes from the plate
  • Each tube clicks into place in the frame ensuring tubes are secure once inserted
  • We have now extended our Random Access range to include PCR plates with optically clear flat bottom wells for microscopy applications
  • The Random Access 96 Well Skirted Optical Bottom PCR Plates (next to their counterpart within our FrameStar® range, 4ti-0970) are currently unique in the market, being suitable for use in both microscopy and PCR, for example, when single cell sorting is followed by molecular biology applications such as qPCR and sequencing
  • Due to their flat bottoms and stackability, these plates are well suited for small sample volume storage (such as compound libraries), with no risk of damaging the seal of the plate below
  • Additionally, the small well volume enables excellent sample recovery
  • The rigid polycarbonate skirt of this design eliminates warping and distortion during PCR
  • Together with the SBS footprint, this makes the plate suitable for use with robotic systems, ideal for both small and high throughput labs
  • Empty frames are available for tubes to be transferred to. The frames can be re-used multiple times, depending on the application
Figure 1. Technical drawing of a conical RA PCR tube (A) compared to a flat bottom RA PCR tube (B)
  • Figure 1 illustrates the differences in tube format between the standard conical PCR tube and the flat bottom tubes
  • The surface area of the optical base is 7.74 mm2, with a thickness of 0.5 mm
  • The total volume of a conical well is 0.2 ml compared to 0.18 ml for flat bottom tubes
  • The primary target application for optical bottom plates is assays that require the use of microscopy, for example, work with single cells including fluorescent imaging and cell sorting
Key Features
  • Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen

Tubes

  • Individually removable from the rigid frame, while the rest of the tubes can remain in storage, reducing freeze-thawing of the whole plate
  • Thin walled tubes are made from PP for optimal results in (q)PCR whilst also being suitable for storage and incubation
  • <100 µl working volume, <180 µl total well capacity

Frame

  • Rigid polycarbonate frame provides mechanical stability of the plate
  • Alphanumeric grid reference to aid well and sample identification

Use

  • Suitable for microscopy and small volume sample storage
  • Ideal for use with robotic systems
  • Fewer transfer steps as the same tube can be used for microscopy, (q)PCR and storage
  • Each tube can still be separated after sealing
  • Each tube can be individually heat sealed using the Random Access Pierce Heat Seal Strong, such as the contents of each tube is left secure from cross contamination and evaporation for storage and transfer

Options

  • Also available as a FrameStar® plate (4ti-0970) with fixed wells
  • Empty frames are available for tubes to be transferred to; frames can be re-used multiple times, depending on the application
  • We also offer customization of the Random Access Plates such as color coding of the individual wells; please contact us for details
  • Available barcoded upon request; learn more about our barcoding options
  • The plate can be sealed in one step using Random Access Pierce Heat Seal Strong (4ti-05381/RA) on the 4s3™ Semi-Automatic Sheet Heat Sealer (using the 4ti-0613 Random Access adapter), resulting in individually sealed tubes with pierceable seals to allow for sample access
Specifications

Technical specifications

Parameter Value
Plate length 127.76 ± 0.25 mm
Plate width 85.48 ± 0.25 mm
Plate height 15.50 ± 0.25 mm
Well depth 12 ± 0.10 mm
Well diameter 5.46 ± 0.10 mm
Distance to center of A1 from top edge 11.24 ± 0.25 mm
Distance to center of A1 from left edge 14.38 ± 0.25 mm
Pitch (distance between A1 and A2) 9.00 mm
Compatibility
  • AGILENT/STRATAGENE
    • Thermal Cyclers (96 well block): Robocycler Gradient
    • qPCR Cyclers (96 well block): AriaMx
  • BIO-RAD
    • Thermal Cyclers (96 well block): C1000 Touch | S1000
    • qPCR Cyclers (96 well block): CFX96 Touch | CFX96 Touch Deep Well | CFX connect
  • BIO-RAD MJ RESEARCH
    • Thermal Cyclers (96 well block): PCT100™/200™/220™/221™/225™/240™
    • qPCR Cyclers (96 well block): Chromo4™ | Opticon2™
  • EPPENDORF
    • Thermal Cyclers (96 well block): MasterCycler® ep/ep gradient/Pro/Pro S/nexus/nexus gradient/nexus SX1/nexus GSX1
    • qPCR Cyclers (96 well block): MasterCycler™ ep realplex
  • PEQLAB/VWR
    • Thermal Cyclers (96 well block): peqSTAR 96X
  • SENSOQUEST
    • Thermal Cyclers (96 well block): Labcycler
  • TECHNE
    • Thermal Cyclers (96 well block): TC412 | TC512 | Genius | Genius Quad | Touchgene | Touchgene Gradient | Flexigene
    • qPCR Cyclers (96 well block): Quantica

For recommended plate options depending on manufacturer, block type, and instrument please refer to the 4titude Plate Instrument Compatibility Table.

Ordering

Product codes

Part number Description
Random Access 96 Well Skirted Optical Bottom PCR Plate
96 individual tubes in plate format, low profile, flat optical bottom, 0.1 ml clear polypropylene tubes, black rigid polycarbonate frame, cut corner H1, working volume: <100 µl, total well capacity: 180 µl
4ti-0970/RA clear tubes, black frame; 50 plates
4ti-0970/RA-F black frame (no wells, frame only); 10 frames