FrameStar® 96 Well Skirted Optical Bottom PCR Plate

Low profile, flat optical bottom, 0.1 ml clear polypropylene wells, clear polycarbonate frame, cut corner H1, working volume: <100 µl, total well capacity: 180 µl

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This product is covered by one or more of the following U.S. patents or their foreign counterparts, owned by Eppendorf AG: U.S. Patent Nos. 7,347,977 and 6,340,589.

Overview

Low profile, flat optical bottom, 0.1 ml clear polypropylene wells, clear polycarbonate frame, cut corner H1, working volume: <100 µl, total well capacity: 180 µl

  • We have now extended our FrameStar® range to include PCR plates with optically clear flat bottom wells for microscopy applications
  • The FrameStar® 96 Well Skirted Optical Bottom PCR Plates (next to their counterpart within our Random Access range, 4ti-0970/RA) are currently unique in the market, being suitable for use in both microscopy and PCR
  • Optical bottom plates are ideal for applications requiring single cell sorting followed by molecular biology techniques such as (q)PCR and sequencing
  • The flat bottoms enable excellent stackability, making these plates well suited for small sample volume storage such as compound libraries, with no risk of damaging the seal of the plate below
  • Additionally, the small well volume enables excellent sample recovery
  • The low profile wells of this plate are shorter than "standard" profile wells, which decreases the "dead space" between the heated lid of the thermal cycler and the reagents in the well
  • This reduces condensation forming on the side wall of the tubes, moderating changes in PCR volume and increasing the efficiency of the reaction
  • This is especially recommended for reaction volumes below 20 µl
  • The rigid polycarbonate skirt of this design eliminates warping and distortion during PCR, and makes it ideal for use with robotic systems
Figure 1. Technical drawing of a conical PCR tube (A) compared to a flat bottom PCR tube (B)
  • Figure 1 illustrates the differences in tube format between the standard conical PCR tube and the flat bottom tubes
  • The surface area of the optical base is 7.74 mm2, with a thickness of 0.5 mm. The total volume of a conical well is 0.2 ml compared to 0.18 ml for flat bottom tubes
  • The primary target application for optical bottom plates is assays that require the use of microscopy, for example, work with single cells including fluorescent imaging and cell sorting

This product is covered by one or more of the following U.S. patents or their foreign counterparts, owned by Eppendorf AG: U.S. Patent Nos. 7,347,977 and 6,340,589.

Key Features

  • Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen
  • Stackable

Wells

  • Ultra-smooth, uniform, thin-walled polypropylene wells for optimum PCR and real-time (RT-qPCR) results
  • <0.1 ml (100 µl) working volume, 0.18 ml (180 µl) total well capacity

Frame

  • Rigid polycarbonate frame for added mechanical stability
  • Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
  • Improved seal integrity due to thermal stability of frame
  • Alphanumeric grid reference to aid well and sample identification

Use

  • Suitable for microscopy and small volume sample storage
  • Recommended for low volume PCR
  • Compatible with standard multichannel pipettes
  • Ideal for use with robotic systems

Options

Specifications

Technical specifications

Parameter Value
Plate length 127.70 ± 0.25 mm
Plate width 85.48 ± 0.25 mm
Plate height 16.10 ± 0.25 mm
Well depth 12 ± 0.10 mm
Well diameter 5.46 ± 0.10 mm
Distance to center of A1 from top edge 11.24 ± 0.25 mm
Distance to center of A1 from left edge 14.38 ± 0.25 mm
Pitch (distance between A1 and A2) 9.00 mm

Compatibility

  • AGILENT/STRATAGENE
    • Thermal Cyclers (96 well block): Robocycler Gradient
    • qPCR Cyclers (96 well block): AriaMx
  • ANALYTIK JENA/BIOMETRA
    • Thermal Cyclers (96 well block): Flexcycler2 | T1 Thermocycler | T1 Thermocycler | Tgradient | Tone | Tadvanced | TProfessional (Standard/Basic) Gradient / XL | Trobot 96 | SpeedCycler2 (SP, SPR)
  • BIO-RAD
    • Thermal Cyclers (96 well block): C1000 Touch | S1000
    • qPCR Cyclers (96 well block): CFX96 Touch | CFX 96 Touch Deep Well | CFX connect
  • BIO-RAD MJ RESEARCH
    • Thermal Cyclers (96 well block): PCT100™/200™/220™/221™/225™/240™
    • qPCR Cyclers (96 well block): Chromo4™ | Opticon2™
  • EPPENDORF
    • Thermal Cyclers (96 well block) : MasterCycler® ep/ep gradient/Pro/Pro S/nexus/nexus gradient/nexus SX1/nexus GSX1
    • qPCR Cyclers (96 well block): MasterCycler™ ep realplex
  • GE HEALTHCARE/AMERSHAM
    • Sequencers (96 well block): MegaBACE™ 500 | MegaBACE™ 1000 mark 2
  • PEQLAB/VWR
    • Thermal Cyclers (96 well block): peqSTAR 96X
  • SENSOQUEST
    • Thermal Cyclers (96 well block): Labcycler
  • TECHNE
    • Thermal Cyclers (96 well block): TC412 | TC512 | Genius | Genius Quad | Touchgene | Touchgene Gradient | Flexigene
    • qPCR Cyclers (96 well block): Quantica

For recommended plate options depending on manufacturer, block type, and instrument please refer to the 4titude Plate Instrument Compatibility Table and look for the FrameStar® 96 Well Skirted PCR Plate (4ti-0960).

Ordering

Product codes

Part number Description
FrameStar® 96 Well Skirted Optical Bottom PCR Plate
Low profile, flat optical bottom, 0.1 ml clear polypropylene wells, clear polycarbonate frame, cut corner H1, working volume: <100 µl, total well capacity: 180 µl
4ti-0970 clear wells, clear frame; 50 plates

This product is covered by one or more of the following U.S. patents or their foreign counterparts, owned by Eppendorf AG: U.S. Patent Nos. 7,347,977 and 6,340,589.