FrameStar® 96 Well Skirted PCR Plate

Low profile, 0.1 ml polypropylene wells, polycarbonate frame, cut corner H1; working volume: <100 µl, total well capacity: 200 µl

Request a Sample

This product is covered by one or more of the following U.S. patents or their foreign counterparts, owned by Eppendorf AG: U.S. Patent Nos. 7,347,977 and 6,340,589.

Overview

Low profile, 0.1 ml polypropylene wells, polycarbonate frame, cut corner H1; working volume: <100 µl, total well capacity: 200 µl

  • The low profile wells of this plate are shorter than "standard" profile wells, which decrease the "dead space" between the heated lid of the thermal cycler and the sample in the well
  • This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction
  • This is especially recommended for reaction volumes below 20 µl
  • The rigid polycarbonate skirt of this design eliminates warping and distortion during PCR, and makes it ideal for use with robotic systems

This product is covered by one or more of the following U.S. patents or their foreign counterparts, owned by Eppendorf AG: U.S. Patent Nos. 7,347,977 and 6,340,589.

Key Features

  • Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen
  • Stackable

Wells

  • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
  • <0.1 ml (100 µl) working volume, 0.2 ml (200 µl) total well capacity

Frame

  • Rigid polycarbonate frame for added mechanical stability
  • Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
  • Improved seal integrity due to thermal stability of frame
  • Alphanumeric grid reference to aid well and sample identification

Use

  • Recommended for low volume PCR
  • Ideal for use with robotic systems
  • Compatible with standard multichannel pipettes

Options

  • Available with the following frame color options for the clear well variety: purple, blue, clear, green, red, black, and white
  • Also available with a black frame and with a clear frame for the white well variety, for optimum signal-to-noise ratio when using fluorescent based assays
  • Also available as a black frame with black wells for non-PCR fluorescent applications
  • Extra rigid skirt option (4ti-0960/RIG) for use with Perkin-Elmer® Sciclone, Beckman, Hamilton, and other automation systems: eliminates the robotic grip picking up more than one plate at a time
  • Ethylene oxide treated option available (4ti-OX960C/SBC) for forensic use; learn more about our ethylene oxide treated range 
  • Ultra-low DNA binding option available (4ti-LB0960/RIG) for sensitive applications with ultra-low DNA input and for maximum DNA recovery after low temperature storage and high temperature incubation; learn more about our low binding range
  • Available barcoded upon request; learn more about our barcoding options
  • Combi packs available (for 4ti-0960) with Q-Stick™ qPCR Seal (4ti-0565)
  • Clear Polystyrene Lid (4ti-0287) available for 4ti-0960/RIG

Specifications

Technical specifications

Parameter Value
Plate length 127.76 ± 0.25 mm
Plate width 85.48 ± 0.25 mm
Plate height 16.10 ± 0.25 mm
Well depth 15.10 ± 0.10 mm
Well diameter 5.50 ± 0.10 mm
Distance to center of A1 from top edge 11.24 ± 0.25 mm
Distance to center of A1 from left edge 14.38 ± 0.25 mm
Pitch (distance between A1 and A2) 9.00 mm

Compatibility

  • AGILENT/STRATAGENE
    • Thermal Cyclers (96 well block): Robocycler Gradient
    • qPCR Cyclers (96 well block): AriaMx
  • ANALYTIK JENA/BIOMETRA
    • Thermal Cyclers (96 well block): Flexcycler2 | T1 Thermocycler | T1 Thermocycler | Tgradient | Tone | Tadvanced | TProfessional (Standard/Basic) Gradient/XL | Trobot 96 | SpeedCycler2 (SP, SPR)
  • BIO-RAD
    • Thermal Cyclers (96 well block): C1000 Touch | S1000
    • qPCR Cyclers (96 well block): CFX96 Touch | CFX 96 Touch Deep Well | CFX connect
  • BIO-RAD MJ RESEARCH
    • Thermal Cyclers (96 well block): PCT100™/200™/220™/221™/225™/240™
    • qPCR Cyclers (96 well block): Chromo4™ | Opticon2™
  • EPPENDORF
    • Thermal Cyclers (96 well block): MasterCycler® ep/ep gradient/Pro/Pro S/nexus/nexus gradient/nexus SX1/nexus GSX1
    • qPCR Cyclers (96 well block): MasterCycler™ ep realplex
  • GE HEALTHCARE/AMERSHAM
    • Sequencers (96 well block): MegaBACE™ 500 | MegaBACE™ 1000 mark 2
  • PEQLAB/VWR
    • Thermal Cyclers (96 well block): peqSTAR 96X
  • SENSOQUEST
    • Thermal Cyclers (96 well block): Labcycler
  • TECHNE
    • Thermal Cyclers (96 well block): TC412 | TC512 | Genius | Genius Quad | Touchgene | Touchgene Gradient | Flexigene
    • qPCR Cyclers (96 well block): Quantica

For recommended plate options depending on manufacturer, block type, and instrument please refer to the 4titude Plate Instrument Compatibility Table.

Ordering

Product codes

Part number Description
FrameStar® 96 Well Skirted PCR Plate
Low profile, 0.1 ml polypropylene wells, polycarbonate frame, cut corner H1; working volume: <100 µl, total well capacity: 200 µl
4ti-0960 clear wells, purple frame; 50 plates
4ti-0960/B clear wells, blue frame; 50 plates
4ti-0960/C clear wells, clear frame; 50 plates
4ti-0960/G clear wells, green frame; 50 plates
4ti-0960/R clear wells, red frame; 50 plates
4ti-0960/X clear wells, black frame; 50 plates
4ti-0960/W clear wells, white frame; 50 plates
4ti-0961 white wells, black frame; 50 plates
4ti-0961/C white wells, clear frame; 50 plates
4ti-0966 black wells, black frame; 50 plates
4ti-OX960C/SBC 4ti-0960/C for forensic use, ethylene oxide treated and single barcoded; 20 plates
4ti-0960/RIG 4ti-0960/X with extra rigid skirt; 50 plates
4ti-LB0960/RIG 4ti-0960/RIG, with low DNA binding properties; 50 plates
Combi packs
4ti-0960/0565 4ti-0960 + Q-Stick™ qPCR Seal (4ti-0565); 50 plates + 50 seals
Lids
4ti-0287 FrameStar® 96 NGS Lid (4ti-0287), for use with 4ti-0960/RIG; 50 lids

This product is covered by one or more of the following U.S. patents or their foreign counterparts, owned by Eppendorf AG: U.S. Patent Nos. 7,347,977 and 6,340,589.