FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style

Low profile, 0.1 ml polypropylene wells, polycarbonate frame, cut corner H12; working volume: <100 µl, total well capacity: 200 µl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block)

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This product is covered by one or more of the following U.S. patents or their foreign counterparts, owned by Eppendorf AG: U.S. Patent Nos. 7,347,977 and 6,340,589.

Overview

Low profile, 0.1 ml polypropylene wells, polycarbonate frame, cut corner H12; working volume: <100 µl, total well capacity: 200 µl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block)

  • Our FrameStar® Roche Style plates are designed to achieve optimized assay conditions on the Roche LightCycler® 96 and 480 (with 96 well block)
  • This particular style of plate is in a low profile 96-well format; perfect for reaction volumes of 10-100 µl
  • The wells are shorter than "standard" profile wells, which decrease the "dead space" between the heated lid of the thermal cycler and the sample in the well
  • This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction
  • This is especially recommended for reaction volumes below 20 µl
  • The semi-skirted frame allows for labeling or barcoding

This product is covered by one or more of the following U.S. patents or their foreign counterparts, owned by Eppendorf AG: U.S. Patent Nos. 7,347,977 and 6,340,589.

Key Features

  • Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen

Wells

  • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
  • <0.1 ml (100 µl) working volume, 0.2 ml (200 µl) total well capacity

Frame

  • Rigid polycarbonate frame for added mechanical stability
  • Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
  • Improved seal integrity due to thermal stability of frame
  • Alphanumeric grid reference to aid well and sample identification

Use

  • Compatible with the Roche LightCycler® 96 and 480 (with 96 well block)
  • Recommended for low volume PCR
  • Ideal for use with robotic systems
  • Compatible with standard multichannel pipettes

Options

  • Standard plate comes with clear polypropylene wells on a clear polycarbonate frame
  • Also available with white wells for optimum signal-to-noise ratio when using fluorescent based assays
  • Available barcoded upon request; learn more about our barcoding options
  • Combi packs available with qPCR Seal (4ti-0560) and (for 4ti-0951) with q-Stick qPCR Seal (4ti-0564)

Specifications

Technical specifications

Parameter Value
Plate length 127.70 ± 0.25 mm
Plate width 85.48 ± 0.25 mm
Plate height 15.60 ± 0.25 mm
Well depth 15.10 ± 0.10 mm
Well diameter 5.50 ± 0.10 mm
Distance to center of A1 from top edge 11.24 ± 0.25 mm
Distance to center of A1 from left edge 14.38 ± 0.25 mm
Pitch (distance between A1 and A2) 9.00 mm

Compatibility

  • ROCHE
    • qPCR Cyclers (96 well block): LC96 | LC480

For recommended plate options depending on manufacturer, block type, and instrument please refer to the 4titude Plate Instrument Compatibility Table.

Ordering

Product codes

Part number Description
FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style
Low profile, 0.1 ml polypropylene wells, polycarbonate frame, cut corner H12; working volume: <100 µl, total well capacity: 200 µl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block)
4ti-0950/C clear wells, clear frame; 50 plates
4ti-0951 white wells, clear frame; 50 plates
Combi packs
4ti-0952 4ti-0951 + qPCR Seal (4ti-0560); 50 plates + 50 seals
4ti-0953 4ti-0950/C + qPCR Seal (4ti-0560); 50 plates + 50 seals
4ti-0951/0565 4ti-0951 + Q-Stick™ qPCR Seal (4ti-0565); 50 plates + 50 seals

This product is covered by one or more of the following U.S. patents or their foreign counterparts, owned by Eppendorf AG: U.S. Patent Nos. 7,347,977 and 6,340,589.