FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style

Low profile, 0.1 ml polypropylene wells, polycarbonate frame, cut corner H12; working volume: <100 µl, total well capacity: 200 µl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block)

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This product is covered by one or more of the following U.S. patents or their foreign counterparts, owned by Eppendorf AG: U.S. Patent Nos. 7,347,977 and 6,340,589.

Overview
  • Our FrameStar® Roche Style plates are designed to achieve optimized assay conditions on the Roche LightCycler® 96 and 480 (with 96 well block)
  • This particular style of plate is in a low profile 96-well format; perfect for reaction volumes of 10-100 µl
  • The wells are shorter than "standard" profile wells, which decrease the "dead space" between the heated lid of the thermal cycler and the sample in the well
  • This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction
  • This is especially recommended for reaction volumes below 20 µl
  • The semi-skirted frame allows for labeling or barcoding

This product is covered by one or more of the following U.S. patents or their foreign counterparts, owned by Eppendorf AG: U.S. Patent Nos. 7,347,977 and 6,340,589.

Key Features
  • Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen
Wells
  • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
  • <0.1 ml (100 µl) working volume, 0.2 ml (200 µl) total well capacity
Frame
  • Rigid polycarbonate frame for added mechanical stability
  • Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
  • Improved seal integrity due to thermal stability of frame
  • Alphanumeric grid reference to aid well and sample identification
Use
  • Compatible with the Roche LightCycler® 96 and 480 (with 96 well block)
  • Recommended for low volume PCR
  • Ideal for use with robotic systems
  • Compatible with standard multichannel pipettes
Options
  • Standard plate comes with clear polypropylene wells on a clear polycarbonate frame
  • Also available with white wells for optimum signal-to-noise ratio when using fluorescent based assays
  • Available barcoded upon request; learn more about our barcoding options
  • Combi packs available with qPCR Seal (4ti-0560) and (for 4ti-0951) with q-Stick qPCR Seal (4ti-0564)
Specifications
Technical specifications
Parameter Value
Plate length 127.70 ± 0.25 mm
Plate width 85.48 ± 0.25 mm
Plate height 15.60 ± 0.25 mm
Well depth 15.10 ± 0.10 mm
Well diameter 5.50 ± 0.10 mm
Distance to center of A1 from top edge 11.24 ± 0.25 mm
Distance to center of A1 from left edge 14.38 ± 0.25 mm
Pitch (distance between A1 and A2) 9.00 mm
Compatibility
  • ROCHE
    • qPCR Cyclers (96 well block): LC96 | LC480

For recommended plate options depending on manufacturer, block type, and instrument please refer to the 4titude Plate Instrument Compatibility Table.

Ordering Information
Product codes

Use these part numbers to request a sample or a quote:

Part number Description
FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style
Low profile, 0.1 ml polypropylene wells, polycarbonate frame, cut corner H12; working volume: <100 µl, total well capacity: 200 µl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block)
4ti-0950/C clear wells, clear frame; 50 plates
4ti-0951 white wells, clear frame; 50 plates
Combi packs
4ti-0952 4ti-0951 + qPCR Seal (4ti-0560); 50 plates + 50 seals
4ti-0953 4ti-0950/C + qPCR Seal (4ti-0560); 50 plates + 50 seals
4ti-0951/0565 4ti-0951 + Q-Stick™ qPCR Seal (4ti-0565); 50 plates + 50 seals

This product is covered by one or more of the following U.S. patents or their foreign counterparts, owned by Eppendorf AG: U.S. Patent Nos. 7,347,977 and 6,340,589.