FrameStar® 96 Well Semi-Skirted PCR Plate, ABI® Style

Standard profile, 0.2 ml polypropylene wells, polycarbonate frame, cut corner A12; working volume: <200 µl, total well capacity: 300 µl; designed for use on all major cyclers, including ABI® instruments with standard 96 well blocks

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This product is covered by one or more of the following U.S. patents or their foreign counterparts, owned by Eppendorf AG: U.S. Patent Nos. 7,347,977 and 6,340,589.

Overview

Standard profile, 0.2 ml polypropylene wells, polycarbonate frame, cut corner A12; working volume: <200 µl, total well capacity: 300 µl; designed for use on all major cyclers, including ABI® instruments with standard 96 well blocks

  • Specifically designed to be directly compatible with all major thermal cyclers, this plate can be used directly in ABI® 96 well instruments without the need for any adapters
  • The rigid FrameStar® 2-component design eliminates warping and distortion during PCR, making it ideal for use with robotic systems
  • The semi-skirt allows for labeling or barcoding for sample tracking
  • Learn more about the features and benefits of the FrameStar® range

This product is covered by one or more of the following U.S. patents or their foreign counterparts, owned by Eppendorf AG: U.S. Patent Nos. 7,347,977 and 6,340,589.

Key Features
  • Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen

Wells

  • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
  • <0.2 ml (200 µl) working volume, 0.3 ml (300 µl) total well capacity

Frame

  • Cut corner at A12
  • Rigid polycarbonate frame for added mechanical stability
  • Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
  • Improved seal integrity due to thermal stability of frame
  • Alphanumeric grid reference to aid well and sample identification

Use

  • Perfect for ABI® thermal cyclers & sequencers
  • Recommended for low volume PCR
  • Ideal for use with robotic systems
  • Compatible with standard multichannel pipettes

Options

  • Available with the following frame color options for the clear well variety: purple, blue, clear, green, red, and black
  • Also available as a black frame with white wells for optimum signal-to-noise ratio when using fluorescent based assays
  • Also available as a clear frame with frosted wells for use with ABI®/LifeTechnologies® qPCR instruments
  • Ultra-low DNA binding option available (4ti-LB0770/C) for sensitive applications with ultra-low DNA input and for maximum DNA recovery after low temperature storage and high temperature incubation; learn more about our low binding range
  • Ethylene oxide treated option available (4ti-OX770C/SBC) for forensic use; learn more about our ethylene oxide treated range 
  • Available barcoded upon request; learn more about our barcoding options
  • FrameStar® 96 Lid (4ti-0289) available
Specifications

Technical specifications

Parameter Value
Plate length 124.26 ± 0.25 mm
Plate width 83.97 ± 0.25 mm
Plate height 20.70 ± 0.25 mm
Well depth 20.20 ± 0.10 mm
Well diameter 5.46 ± 0.10 mm
Distance to center of A1 from top edge 10.495 ± 0.25 mm
Distance to center of A1 from left edge 12.63 ± 0.25 mm
Pitch (distance between A1 and A2) 9.00 mm
Compatibility
  • ABI®/LIFE TECHNOLOGIES/THERMO FISHER SCIENTIFIC
    • Thermal Cyclers (96 well standard block): Verity | Proflex | Simpliamp | GeneAmp® 2700/2720/9600/9700
    • qPCR Cyclers (96 well standard block): 7000 | 7300 | 7500 | 7700 | 7900 HT | QuantStudio™ 3/5/6/7/12K | ViiA7™
    • Sequencers (96 well block): 3100 | 3130XL | 3500 | 3500XL | 3730 | 3730XL
  • AGILENT/STRATAGENE
    • qPCR Cyclers (96 well block): Mx3000P™ | Mx3005P™ | Mx4000P™
  • ANALYTIK JENA/BIOMETRA
    • Thermal Cyclers (96 well block): Flexcycler2 | T1 Thermocycler | Tgradient | Tone | Tadvanced | TProfessional (Standard/Basic) Gradient / XL | Trobot 96 | SpeedCycler2 (SP, SPR)
    • qPCR Cyclers (96 well block): qTOWER3/G/touch | Toptical
  • BIOER TECHNOLOGIES
    • Thermal Cyclers (96 well block): Gene Touch 96
  • BIO-RAD
    • Thermal Cyclers (96 well block): C1000 Touch | S1000 | iCycler™ | MyCycler™ | T100
    • qPCR Cyclers (96 well block): MyiQ™ | iCycler™ IQ/IQ 4/IQ 5
  • BIO-RAD MJ RESEARCH
    • Thermal Cyclers (96 well block): PCT100™/200™/220™/221™/225™/240™
    • qPCR Cyclers (96 well block): Chromo4™
  • CORBETT RESEARCH
    • Thermal Cyclers (96 well block): (Qiagen) Palm Cycler
  • EPPENDORF
    • Thermal Cyclers (96 well block): MasterCycler® ep/ep gradient/Pro/Pro S/nexus/nexus gradient/nexus SX1/nexus GSX1
    • qPCR Cyclers (96 well block): Mastercycler™ e realplex
  • PEQLAB/VWR
    • Thermal Cyclers (96 well block): peqSTAR 96X
  • SENSOQUEST
    • Thermal Cyclers (96 well block): Labcycler
  • TAKARA
    • Thermal Cyclers (96 well block): Dice touch | Gradient
  • TECHNE
    • Thermal Cyclers (96 well block): Prime | PrimeG | Prime Elite | Prime Elite Satellite | PCRmax Alpha cycler 1/2/4 | TC412 | TC512 | Genius | Genius Quad | Touchgene | Touchgene Gradient | Flexigene

For recommended plate options depending on manufacturer, block type, and instrument please refer to the 4titude Plate Instrument Compatibility Table.

Ordering

Product codes

Part number Description
FrameStar® 96 Well Semi-Skirted PCR Plate, ABI® Style
Standard profile, 0.2 ml polypropylene wells, polycarbonate frame, cut corner A12; working volume: <200 µl, total well capacity: 300 µl; designed for use on all major cyclers, including ABI® instruments with standard 96 well blocks
4ti-0770 clear wells, purple frame; 50 plates
4ti-0770/B clear wells, blue frame; 50 plates
4ti-0770/C clear wells, clear frame; 50 plates
4ti-0770/C/10P clear wells, clear frame; 10 plates
4ti-0770/G clear wells, green frame; 50 plates
4ti-0770/R clear wells, red frame; 50 plates
4ti-0770/X clear wells, black frame; 50 plates
4ti-0771 white wells, black frame; 50 plates
4ti-0772 frosted wells, clear frame; 50 plates
4ti-0772/10P frosted wells, clear frame; 10 plates
4ti-OX770C/SBC 4ti-0770/C for forensic use, ethylene oxide treated, single barcoded; 20 plates
4ti-LB0770/C 4ti-0770/C, with low DNA binding properties; 50 plates
Lids
4ti-0289 FrameStar® 96 Lid (4ti-0289), for use with the FrameStar® 96 Well Semi-Skirted Plates, ABI® Style range; 50 lids

This product is covered by one or more of the following U.S. patents or their foreign counterparts, owned by Eppendorf AG: U.S. Patent Nos. 7,347,977 and 6,340,589.