96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style

Standard profile, 0.2 ml wells, polypropylene, cut corner A12, working volume: <200 µl, total well capacity: 300 µl; designed for use on ABI® thermal cyclers

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Overview

Standard profile, 0.2 ml wells, polypropylene, cut corner A12, working volume: <200 µl, total well capacity: 300 µl; designed for use on ABI® thermal cyclers

  • We recommend this semi-skirted plate for use with ABI® thermal cyclers and sequencers
  • It can be used directly with ABI® instruments with no adapters and no re-calibration of the instruments necessary
  • The only case where this is not true is with the ABI Fast Block thermal cyclers, in which case using our FrameStar® FastPlate is recommended instead
  • All of our PCR plates are molded from virgin polypropylene under ISO certified cleanroom conditions in our UK-based production facility, and as such comply with the same stringent requirements as our FrameStar® range
Key Features
  • Subject to a strict QC procedure which includes a visual check and leak testing of every tube
  • Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen

Wells

  • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time qPCR results
  • Standard 0.2 ml (200 µl) working volume, with a 0.3 ml (300 µl) total well capacity when used with sealing options
  • Maximum thermal conductivity for efficient heat transfer and precise thermal cycling
  • Raised well rims prevent cross contamination and facilitate effective sealing for reduced evaporation

Frame

  • Alphanumeric grid reference to aid well and sample identification

Use

  • Designed for and compatible with ABI® thermal cyclers and Real Time PCR instruments
  • Compatible with standard multichannel pipettes

Options

  • Clear version has super clear wells for maximum sample visibility
  • Also available with frosted wells for increased qPCR signal intensities and improved detection sensitivity
  • Available barcoded upon request; learn more about our barcoding options
  • Available as a FrameStar® 2-component PCR plate with the same dimensions, but with the added benefits of a polycarbonate frame that eliminates plate distortion and warping during the PCR process, leading to less evaporation and sample loss
Specifications

Technical specifications

Parameter Value
Plate length 126.00 ± 0.25 mm
Plate width 86.00 ± 0.25 mm
Plate height 22.10 ± 0.25 mm
Well depth 20.20 ± 0.10 mm
Well diameter 5.46 ± 0.10 mm
Distance to center of A1 from top edge 11.50 ± 0.10 mm
Distance to center of A1 from left edge 13.50 ± 0.10 mm
Pitch (distance between A1 and A2) 9.00 mm
Compatibility
  • ABI®/LIFE TECHNOLOGIES/THERMO FISHER SCIENTIFIC
    • Thermal Cyclers (96 well standard block): Veriti | Proflex | Simpliamp | GeneAmp® 2700/2720/9600/9700
    • qPCR Cyclers (96 well standard block): 7000 | 7300 | 7500 | 7700 | 7900 HT | QuantStudio™ 3/5/6/7/12K | ViiA7™
    • Sequencers (96 well block): 3100 | 3130XL | 3500 | 3500XL | 3730 | 3730XL
  • SENSOQUEST
    • Thermal Cyclers (96 well block): Labcycler

For recommended plate options depending on manufacturer, block type, and instrument please refer to the 4titude Plate Instrument Compatibility Table.

Ordering

Product codes

Part number Description
96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style
Standard profile, 0.2 ml wells, polypropylene, cut corner A12, working volume: <200 µl, total well capacity: 300 µl; designed for use on ABI® thermal cyclers
4ti-0735 clear; 50 plates
4ti-0736 frosted; 50 plates