FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity

Low profile, 0.1 ml polypropylene wells, polycarbonate frame, cut corner H12, working volume: <100 µl, total well capacity: 200 µl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block); extra white wells for improved sensitivity in fluorescent assays

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This product is covered by one or more of the following U.S. patents or their foreign counterparts, owned by Eppendorf AG: U.S. Patent Nos. 7,347,977 and 6,340,589.

Overview

Low profile, 0.1 ml polypropylene wells, polycarbonate frame, cut corner H12, working volume: <100 µl, total well capacity: 200 µl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block); extra white wells for improved sensitivity in fluorescent assays

  • Our FrameStar® Roche Style plates are designed to achieve optimized assay conditions on the Roche LightCycler® 96 and 480 (with 96 well block)
  • This particular style of plate is in a low profile 96-well format; perfect for reaction volumes of 10-100 µl
  • The wells are shorter than "standard" profile wells, which decrease the "dead space" between the heated lid of the thermal cycler and the sample in the well
  • This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction
  • This is especially recommended for reaction volumes below 20 µl
  • The extra white wells allow for increased sensitivity in fluorescent assays
  • The semi-skirted frame allows for labeling or barcoding

This product is covered by one or more of the following U.S. patents or their foreign counterparts, owned by Eppendorf AG: U.S. Patent Nos. 7,347,977 and 6,340,589.

Key Features

  • Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen

Wells

  • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
  • <0.1 ml (100 µl) working volume, 0.2 ml (200 µl) total well capacity

Frame

  • Rigid polycarbonate frame for added mechanical stability
  • Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
  • Improved seal integrity due to thermal stability of frame
  • Alphanumeric grid reference to aid well and sample identification

Use

  • Compatible with the Roche LightCycler® 96 and 480 (with 96 well block)
  • Recommended for low volume PCR
  • Compatible with standard multichannel pipettes
  • Ideal for use with robotic systems

Options

  • Plates are available with a clear polycarbonate frame and extra white wells for improved sensitivity when using fluorescent based assays
  • Combi packs available with qPCR Seal (4ti-0560)
  • Available barcoded upon request; learn more about our barcoding options

Specifications

Technical specifications

Parameter Value
Plate length 127.70 ± 0.25 mm
Plate width 85.48 ± 0.25 mm
Plate height 15.60 ± 0.25 mm
Well depth 15.10 ± 0.10 mm
Well diameter 5.50 ± 0.10 mm
Distance to center of A1 from top edge 11.24 ± 0.25 mm
Distance to center of A1 from left edge 14.38 ± 0.25 mm
Pitch (distance between A1 and A2) 9.00 mm

Ct values comparison

  • Ct values of data run on a Roche LightCycler® 480, comparing 4titude FrameStar® Roche Style, High Sensitivity plates (4ti-0954) and Competitor R equivalent plates
  • The new 4titude 4ti-0954 plate out performs the competitor plate with significantly earlier Ct value
FrameStar® Roche Style, High Sensitivity Plate
Ct value 17.23
Δ Ct -3.05
Competitor R equivalent plates
Ct value 20.28
Δ Ct 0

Compatibility

  • ROCHE
    • qPCR Cyclers (96 well block): LC96 | LC480

For recommended plate options depending on manufacturer, block type, and instrument please refer to the 4titude Plate Instrument Compatibility Table.

Ordering

Product codes

Part number Description
FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity Plate
Low profile, 0.1 ml polypropylene wells, polycarbonate frame, cut corner H12, working volume: <100 µl, total well capacity: 200 µl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block); extra white wells for improved sensitivity in fluorescent assays
4ti-0954 extra white wells, clear frame; 50 plates
Combi packs
4ti-0954/0560 4ti-0954 + qPCR Seal (4ti-0560); 50 plates + 50

This product is covered by one or more of the following U.S. patents or their foreign counterparts, owned by Eppendorf AG: U.S. Patent Nos. 7,347,977 and 6,340,589.