FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity
Low profile, 0.1ml polypropylene wells, polycarbonate frame, cut corner H12, working volume: <100µl, total well capacity: 200µl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block); extra white wells for improved sensitivity in fluorescent assays
This product is covered by one or more of the following U.S. patents or their foreign counterparts, owned by Eppendorf AG: U.S. Patent Nos. 7,347,977 and 6,340,589.
- Our FrameStar® Roche Style plates are designed to achieve optimized assay conditions on the Roche LightCycler® 96 and 480 (with 96 well block)
- This particular style of plate is in a low profile 96-well format; perfect for reaction volumes of 10-100µl
- The wells are shorter than "standard" profile wells, which decrease the "dead space" between the heated lid of the thermal cycler and the sample in the well
- This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction
- This is especially recommended for reaction volumes below 20µl
- The extra white wells allow for increased sensitivity in fluorescent assays
- The semi-skirted frame allows for labeling or barcoding
This product is covered by one or more of the following U.S. patents or their foreign counterparts, owned by Eppendorf AG: U.S. Patent Nos. 7,347,977 and 6,340,589.
- Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen
- Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
- <0.1ml (100µl) working volume, 0.2ml (200µl) total well capacity
- Rigid polycarbonate frame for added mechanical stability
- Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
- Improved seal integrity due to thermal stability of frame
- Alphanumeric grid reference to aid well and sample identification
- Compatible with the Roche LightCycler® 96 and 480 (with 96 well block)
- Recommended for low volume PCR
- Compatible with standard multichannel pipettes
- Ideal for use with robotic systems
- Plates are available with a clear polycarbonate frame and extra white wells for improved sensitivity when using fluorescent based assays
- Combi packs available with qPCR Seal (4ti-0560)
- Available barcoded upon request; learn more about our barcoding options
Parameter | FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity |
Plate length | 127.70 ± 0.25mm |
Plate width | 85.48 ± 0.25mm |
Plate height | 15.60 ± 0.25mm |
Well depth | 15.10 ± 0.10mm |
Well diameter | 5.50 ± 0.10mm |
Total well capacity | 0.2ml (200µl) |
Working volume | <0.1ml (100µl) |
Distance to center of A1 from top edge | 11.24 ± 0.25mm |
Distance to center of A1 from left edge | 14.38 ± 0.25mm |
Pitch (distance between A1 and A2) | 9.00mm |
Ct values comparison
- Ct values of data run on a Roche LightCycler® 480, comparing 4titude FrameStar® Roche Style, High Sensitivity plates (4ti-0954) and Competitor R equivalent plates
- The new 4titude 4ti-0954 plate out performs the competitor plate with significantly earlier Ct value
FrameStar® Roche Style, High Sensitivity Plate | |
Ct value | 17.23 |
Δ Ct | -3.05 |
Competitor R equivalent plates | |
Ct value | 20.28 |
Δ Ct | 0 |
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ROCHE
- qPCR Cyclers (96 well block): LC96 | LC480
For recommended plate options depending on manufacturer, block type, and instrument please refer to the 4titude Plate Instrument Compatibility Table.
Use these part numbers to request a quote, a sample or to contact an expert:
Part number | Description |
FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity Plate Low profile, 0.1ml polypropylene wells, polycarbonate frame, cut corner H12, working volume: <100µl, total well capacity: 200µl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block); extra white wells for improved sensitivity in fluorescent assays |
|
4ti-0954 | extra white wells, clear frame; 50 plates |
Combi packs | |
4ti-0954/0560 | 4ti-0954 + qPCR Seal (4ti-0560); 50 plates + 50 seals |
This product is covered by one or more of the following U.S. patents or their foreign counterparts, owned by Eppendorf AG: U.S. Patent Nos. 7,347,977 and 6,340,589.