96 Well Semi-Skirted PCR Plate, Roche Style

Low profile, 0.1 ml wells, polypropylene, cut corner H12, working volume: <100 µl, total well capacity: 200 µl; designed for use on Roche LightCycler 480®

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Overview

  • Low profile, 0.1 ml wells, polypropylene, cut corner H12, working volume: <100 µl, total well capacity: 200 µl; designed for use on Roche LightCycler 480®
  • This Roche Style plate is designed to achieve optimized assay conditions on the Roche LightCycler® 480
  • This particular style of plate is in a low profile 96-well format, perfect for reaction volumes of 10-100 µl
  • The wells are shorter than "standard" profile wells, which decrease the "dead space" between the heated lid of the thermal cycler and the sample in the well
  • This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction
  • This is especially recommended for reaction volumes below 20 µl

Key Features

  • Subject to a strict QC procedure which includes a visual check and leak testing of every tube
  • Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen

Wells

  • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
  • <0.1 ml (100 µl) working volume, 0.2 ml (200 µl) total well capacity
  • Maximum thermal conductivity for efficient heat transfer and precise thermal cycling

Frame

  • Alphanumeric grid reference to aid well and sample identification

Use

  • Optimized for use with the Roche LightCycler® 480
  • Recommended for low volume PCR
  • Compatible with standard multichannel pipettes

Options

  • Available with white wells give optimum signal-to-noise ratio when using fluorescent-based assays
  • Combi packs available with qPCR Seal (0560)
  • Available barcoded upon request; learn more about our barcoding options
  • Available as a FrameStar® 2-component PCR plate with the same dimensions, but with the added benefits of a polycarbonate frame that eliminates plate distortion and warping during the PCR process, leading to less evaporation and sample loss

Specifications

Technical specifications

Parameter Value
Plate length 127.70 ± 0.25 mm
Plate width 85.48 ± 0.25 mm
Plate height 15.60 ± 0.10 mm
Well depth 15.10 ± 0.10 mm
Well diameter 5.50 ± 0.10 mm
Distance to center of A1 from top edge 11.24 ± 0.25 mm
Distance to center of A1 from left edge 14.38 ± 0.25 mm
Pitch (distance between A1 and A2) 9.00 mm

Compatibility

  • ROCHE
    • qPCR Cyclers (96 well block): LC96 | LC480

For recommended plate options depending on manufacturer, block type, and instrument please refer to the 4titude Plate Instrument Compatibility Table.

Ordering

Product codes

Part number Description
96 Well Semi-Skirted PCR Plate, Roche Style
Low profile, 0.1 ml wells, polypropylene, cut corner H12, working volume: <100 µl, total well capacity: 200 µl; designed for use on Roche LightCycler 480®
4ti-0955 white; 50 plates
Combi packs
4ti-0955/0560 4ti-0955 + qPCR Seal (4ti-0560); 50 plates + 50 seals

Resources

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