96 Well Semi-Skirted PCR Plate

Standard profile, 0.2 ml wells, polypropylene, cut corner A12, working volume: <200 µl, total well capacity: 300 µl; universal semi-skirted plate, designed for use on standard thermal cyclers

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Overview

Standard profile, 0.2 ml wells, polypropylene, cut corner A12, working volume: <200 µl, total well capacity: 300 µl; universal semi-skirted plate, designed for use on standard thermal cyclers

  • All of our PCR plates are molded from virgin polypropylene under ISO certified cleanroom conditions in our UK-based production facility, and as such comply with the same stringent requirements as our FrameStar® range.

Key Features

  • Subject to a strict QC procedure which includes a visual check and leak testing of every tube
  • Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen

Wells

  • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
  • Standard 0.2 ml (200 µl) working volume, with a 0.3 ml (300 µl) total well capacity when used with sealing options
  • Maximum thermal conductivity for efficient heat transfer and precise thermal cycling
  • Raised well rims prevent cross contamination and facilitate effective sealing for reduced evaporation

Frame

  • Alphanumeric grid reference to aid well and sample identification

Use

  • Compatible with universal standard block thermal cyclers and sequencers
  • Compatible with standard multichannel pipettes

Options

  • Super clear wells maximize sample visibility
  • Also available as a white plate, ideal for qPCR, giving optimal signal-to-noise ratio for fluorescence-based assays
  • Available barcoded upon request; learn more about our barcoding options
  • Available as a FrameStar® 2-component PCR plate with the same dimensions, but with the added benefits of a polycarbonate frame that eliminates plate distortion and warping during the PCR process, leading to less evaporation and sample loss

Specifications

Technical specifications

Parameter Value
Plate length 124.42 ± 0.25 mm
Plate width 84.02 ± 0.25 mm
Plate height 20.70 ± 0.25 mm
Well depth 20.20 ± 0.10 mm
Well diameter 5.46 ± 0.10 mm
Distance to center of A1 from top edge 10.51 ± 0.10 mm
Distance to center of A1 from left edge 12.71 ± 0.10 mm
Pitch (distance between A1 and A2) 9.00 mm

Compatibility

  • ABI®/LIFE TECHNOLOGIES/THERMO FISHER SCIENTIFIC
    • Thermal Cyclers (96 well standard block): Veriti | Proflex | Simpliamp | GeneAmp® 2700/2720/9600/9700
    • qPCR Cyclers (96 well standard block): 7000 | 7300 | 7500 | 7700 | 7900 HT | QuantStudio™ 3/5/6/7/12K | ViiA7™
    • Sequencers (96 well standard block): 3100 | 3130XL | 3500 | 3500XL | 3730 | 3730XL
  • AGILENT/STRATAGENE
    • qPCR Cyclers (96 well block): Mx3000P™ | Mx3005P™ | Mx4000™
  • ANALYTIK JENA/BIOMETRA
    • Thermal Cyclers (96 well block): Flexcycler2 | T1 Thermocycler | T1 Thermocycler | Tgradient | Tone | Tadvanced | TProfessional (Standard/Basic) Gradient/XL | Trobot 96 | SpeedCycler2 (SP, SPR)
    • qPCR Cyclers (96 well block): qTOWER3/G/touch | Toptical
  • BIOER TECHNOLOGIES
    • Thermal Cyclers (96 well block): Gene Touch 96
  • BIO-RAD
    • Thermal Cyclers (96 well block): C1000 Touch | S1000 | iCycler™ | MyCycler™ | T100
    • qPCR Cyclers (96 well block): MyiQ™ | iCycler IQ™/IQ 4/IQ 5
  • BIO-RAD MJ RESEARCH
    • Thermal Cyclers (96 well block): PCT100™/200™/220™/221™/225™/240™
    • qPCR Cyclers (96 well block): Chromo4™
  • CORBETT RESEARCH
    • Thermal Cyclers (96 well block): (Qiagen) Palm Cycler
  • EPPENDORF
    • Thermal Cyclers (96 well block): MasterCycler® ep/ep gradient/Pro/Pro S/nexus/nexus gradient/nexus SX1/nexus GSX1
    • qPCR Cyclers (96 well block): MasterCycler™ ep realplex
  • PEQLAB/VWR
    • Thermal Cyclers (96 well block): peqSTAR 96X
  • SENSOQUEST
    • Thermal Cyclers (96 well block): Labcycler
  • TAKARA
    • Thermal Cyclers (96 well block): Dice touch | Gradient
  • TECHNE
    • Thermal Cyclers (96 well block): Prime | PrimeG | Prime Elite | Prime Elite Satellite | PCRmax Alpha cycler 1/2/4 | TC412 | TC512 | Genius | Genius Quad | Touchgene | Touchgene Gradient | Flexigene

For recommended plate options depending on manufacturer, block type, and instrument please refer to the 4titude Plate Instrument Compatibility Table.

Ordering

Product codes

Part number Description
96 Well Semi-Skirted PCR Plate
Standard profile, 0.2 ml wells, polypropylene, cut corner A12, working volume: <200 µl, total well capacity: 300 µl; universal semi-skirted plate, designed for use on standard thermal cyclers
4ti-0760 clear; 50 plates
4ti-0761 white; 50 plates
Cap strips
4ti-0751 Strips of 8 Flat Optical Caps; 300 strips
4ti-0752 Strips of 8 Domed Caps; 300 strips