96 Well Assay Plate, Pyramid Flat Bottom

1.1 ml square wells, pyramid flat bottom, polystyrene, cut corner H12

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Overview

1.1 ml square wells, pyramid flat bottom, polystyrene, cut corner H12

  • This plate has a unique shape of the well bottom with a very flat central part
  • The pyramid shape allows for a lower dead volume when used in automation
  • Centrifugation leads to a pellet in the central part of the well rather than a corner
  • The center of the pyramid shape well bottom is very flat, so absorbance measurements would not be compromised
  • We are offering the plate in clear, black and white polystyrene while black plates are suitable for fluorescent applications
  • Most readers measure absorbance by passing light directly through the sample either bottom to top or top to bottom
  • The clear plates are suitable for both top and bottom reading modes, while black and white plates can only be used for top reading mode
  • The pyramid bottom shape results in a very flat optical window that shows good transmission for wavelengths above 310 nm (refers to the clear plate 4ti-0255)
  • The plates base is clear from around 340 nm so would not interfere with optical density reading which are usually performed around 600 nm
  • The high background below 340 nm would preclude their use in DNA or protein work in the UV range
Fig 1. Well bottom shape detail
Fig 1. Well bottom shape detail
Fig 2. Spectral transmission measured in a CLARIOstar (with kind permission from BMG Labtech)
Fig 2. Spectral transmission measured in a CLARIOstar (with kind permission from BMG Labtech)
Fig 3. 3D well scan measured in a CLARIOstar, well scan data of well D06 (with kind permission from BMG Labtech)
Fig 3. 3D well scan measured in a CLARIOstar, well scan data of well D06 (with kind permission from BMG Labtech)
Key Features
  • Made from polystyrene, a hard material with optical clarity
  • Leak-free
  • Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen

Wells

  • Pyramid flat bottom wells, suitable for imaging and cell culture applications
  • Rounded square wells, for best use of space and improvement in sample mixing, suitable for small volumes

Frame

  • Alphanumeric grid reference to aid well and sample identification
  • SBS footprint

Use

  • Best used for cell culture, fluorescence, luminescence, imaging and light assays, ELISA, and homogeneous assays
  • Recommended for top reading fluorescence instrumentation
  • Compatible with plate readers and ideal for use with automation

Options

  • Available as a black plate, for low background fluorescence and minimum light scattering
  • Also available as a white microplate, to give the best results for luminescence detection as it maximizes signal intensity
  • The clear plate offers the best solution for absorption, ELISA, spectophrotometric and storage applications, recommended for imaging
  • Non-sterile as standard; sterilization available upon request
  • Available barcoded upon request; learn more about our barcoding options
Ordering

Product codes

Part number Description
96 Well Assay Plate, Pyramid Flat Bottom
1.1 ml square wells, pyramid flat bottom, polystyrene, cut corner H12
4ti-0265 black; 21 plates
4ti-0275 white; 21 plates
4ti-0255 clear; 21 plates
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