CryoPod™ Carrier Simplifies Ultra-Low Temperature Management

In a recent independent publication discussing best practices for handling cryopreserved cells, researchers cited Brooks Life Sciences CryoPod™ Carrier as an effective tool to prevent temperature excursions when stocking and retrieving frozen biological samples1.

Cryopreservation allows researchers to store biological samples for years without compromising sample integrity. However, biological samples must be collected, processed and stored using best practices to maintain optimal sample quality. It is very difficult to generate robust, reproducible data without high-quality samples2. The quality of cryopreserved samples is affected by both storage temperature and sample handling procedures1,2.

Cryopreserved samples must be kept at temperatures lower than -130°C to prevent sample breakdown from enzymatic reactions4. Every temperature increase of 7.8°C doubles the rate of deleterious chemical reactions inside cryopreserved samples5. Temperature excursions above -130°C can lower the quality of cryopreserved samples. Temperature excursions can occur when cryopreserved samples are added to or retrieved from freezers or when samples are shipped. During these times, samples can be exposed to rapid heating and cooling cycles, which can adversely affect cellular integrity and function.

Researchers can avoid temperature excursions by following best sample handling practices and by validating all procedures and equipment used to handle biological samples. A recent paper written by Frank Simione, founding member and past President of the International Society for Biological and Environmental Repositories3 and Tim Sharpe, Global Director, Biorepository at ICON Laboratory Services discusses best practices when storing and shipping cryopreserved cells1. Simione and Sharpe have decades of experience managing biological samples and biorepositories.

In their paper, they state that liquid nitrogen vapor-phase storage units are the preferred method to store cryopreserved samples because vapor storage is more cost-effective and reliable than mechanical freezers1. They recommend measuring and validating the temperature at the top of each freezer used to store any cryopreserved samples. Freezer temperatures should be equal to or below -150°C for long term storage6.  The authors also cite a paper showing that vapor-phase storage is safer than liquid nitrogen storage because it reduces the risks of sample containers exploding and laboratory staff being exposed to extremely cold liquid7.

Simione and Sharpe also state that Brooks Life Sciences’ CryoPod™ Carrier can be used to prevent temperature excursions when stocking and retrieving cryopreserved samples1. The CryoPod Carrier is a lightweight, portable liquid nitrogen vapor-based device. It reliably holds samples at -150°C or colder for over four hours. The CryoPod Carrier displays and logs temperature, date and time, and features audible and visual alarms to warn if the temperature exceeds a user-defined range.

An optional CryoPod LN2 Filling Station enables single-button, hands-free, liquid nitrogen filling of the CryoPod Carrier in under fifteen minutes. The filling station can connect to existing liquid nitrogen supply.

The CryoPod Carrier and CryoPod LN2 Filling Station can be used as part of an integrated cold chain solution to maintain the cellular viability and function required for regulatory-compliant preclinical and clinical studies.

References

  1. Simione, F and Sharpe, T. Best practices for storing and shipping cryopreserved cells. In Vitro Cell. Dev. Biol. 2017
  2. NCI Best Practices for Biospecimen Resources
  3. International Society for Biological and Environmental Repositories
  4. Mazur, P. Freezing of living cells: mechanisms and implications. Am J Phys. 1984
  5. Ogden, S. Temperature, relative humidity, light, and air quality: basic guidelines for preservation. Northeast Document Conservation Center Preservation Leaflets 2.1. 2007
  6. Simione F. and Karpinsky, J. Z. Points to consider before validating a liquid nitrogen freezer. In: Validation practices for biotechnology products, ASTM STP 1260 American Society for Testing and Materials. 1996
  7. Tedder, R. et al. Hepatitis B transmission from contaminated cryopreservation tank. Lancet. 1995