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Overview

A biobank stores biological samples that give researchers the power to study molecular characteristics of actual disease – then correlate those patterns with what is known about clinical progression. Researchers use samples to:

  • Identify and validate drug targets
  • Target disease mechanisms
  • Develop screening tests for biomarkers linked to disease sub-types
  • Group patients based on genetic characteristics and likelihood of positive response to testing of new drugs
  • Group patients based on disease biomarkers to determine appropriate treatment
Typical Pathway


8a_biobankingsamplemanagement.jpg

Brooks Solutions

Pathway 1.jpg    Patient

  • Biological sample collected from patient within specific biologic context
  • Sample annotated and stored in a controlled environment for secure temperature-safe shipment

Pathway 2.jpg    Medical/Surgical Procedures

  • Change the biologic context of the sample to understand resulting changes in the molecular profiles
  • Study objectives guide procedures and right testing helps ensure desired results

Pathway 3.jpg    Acquisition

  • Adherence to consistency and best practices maintains sample integrity
  • Unique labeling with 2D-barcoded tubes identifies samples

Pathway 3.jpg    Handling/Processing

  • Preparation variables introduced before and during biological stabilization of the sample include time at-room temperature, temperature of the room, type of fixative, time in fixative, method and rate of freezing, and size of aliquots
  • Success of downstream applications based on material, preservative, processing, and required derivatives

Pathway 3.jpg    Storage

  • Storage temperature and duration may impact sample quality if dehydration, desiccation, or oxidation occur
  • Consider container, temperature, time and expected retrieval needs for downstream assays
  • A quality sample tube is paramount as the first line of defense for sample integrity
  • Remove frost as needed to read all tubes on a whole rack reader

Pathway 3.jpg    Distribution

  • Scan the sample out of the collection when it leaves the biobank
  • Account for possible variations in transport that could cause alterations in the sample
  • Collection and shipping kits help maintain consistency and reproducibility with full traceability of each component

Pathway 3.jpg    Scientific Analysis

  • Even with a single analytic method, each investigator and technician involved in sample analysis may contribute to analysis variation
  • Establish baseline sample characterization, integrate biomarker results, and perform quality checks for test and controls to avoid extreme variation
  • Share pre-analytical variables, accessibility rules, and timely information with research collaborators